Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus sp.JM1

查看全文 作　　者：ZHU [1]Yanbing;LI [2]Hebin;ZHANG [1]Xuqin;ZHANG [1]Chunyan;XIANG [1]Jionghua;LIU [1]Guangming

机构地区：[1]School of Biotechnology Engineering, Jimei University, Xiamen 361021, China;[2]Xiamen Medical College, Xiamen 361008, China

出　　处：《Acta Oceanologica Sinica》索引2011年第30卷第6期,共9页

基　　金：The Natural Science Foundation of Fujian Province,China under contract Nos 2008J0067 and 2009J01033;the Program for New Century Excellent Talents in Fujian Province University under contract No.NCETFJ-2007;the Foundation for Innovative Research Team of Jimei University under contract No.2010A005 摘　　要：A thermostable superoxide dismutase (SOD) from the inshore thermophile Thermus sp. JM1 was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1 656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli (E. coli). The prepared apo-enzyme of the purified recombinant SOD (rSOD) was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2+ - reconstituted rSOD (Mn-rSOD) exhibited the specific activity of 1 598 U/mg. SOD from Thermus sp. JM1 was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 11.0. 关键词：锰超氧化物歧化酶栖热菌耐高温 SEPHAROSE 嗜热近岸 SOD基因温泉

相关文献

参考文献（36）
引证文献（1）
耦合文献（0）

参考文献(36)

引证文献(1)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus sp.JM1

参考文献(36)

引证文献(1)

用户提示