Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis

查看全文 作　　者：Abd El-Latif [1,2]Hesham;Rong [1]Qi;Min [1]Yang

机构地区：[1]State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;[2]Genetics Department, Faculty of Agriculture, Assiut University, Assiut, Egypt

出　　处：《Journal of Environmental Sciences》索引2011年第23卷第12期,共6页

基　　金：supported by the National Research Foundation, China (No. 20921140094);the Chinese Academy of Sciences, Knowledge innovation Project(No. KSCX2-YW-G-054, KZCX2-YW-JC407);CASTWAS Postdoctoral Fellowships 2009 摘　　要：The combination of PCR amplification of 16S rRNA genes with denaturing gradient gel electrophoresis (DGGE) analysis was used to reveal the compositions and dynamics of bacterial communities in a sewage treatment plant with two systems, i.e., an anoxic- anaerobic-aerobic system (inverted A2O) and an anaerobic-anoxic-aerobic one (conventional A2O) over a period from February to July 2009, during which both systems experienced serious sludge bulking problems. The DGGE patterns showed that there were many common bands in both systems, suggesting the high similarity of bacterial communities of the two systems. Meanwhile, the moving window correlation analysis showed that the two systems experienced different microbial community structure changes during the period, which might be related with the different situations of the occurrence and disappearance of sludge bulking, as being reflected by sludge volume index (SVI) values. Major bands of DGGE patterns of sludge samples were further sequenced. Phylogenetic affiliation indicated that the majority of the sequences obtained were affiliated with Actinobacteria, Firmicutes, Bacteroidetes/Chlorobi group and α- and β-Proteobacteria. Two sequences showed high similarities to typical filamentous bacteria Microthrix parvicella and Nostocoida limicola I, indicating that these bacterial species have been involved in the sludge bulking problems. 关键词：PCR-DGGE 污水处理厂细菌群落系统群落结构技术分析变性梯度凝胶电泳污泥膨胀

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Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis

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