Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions

查看全文 作　　者：Xiaodong Yuan Yanan Cai Ya Ou Yanhui [1]Lu

机构地区：[1]Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China

出　　处：《Neural Regeneration Research》索引2011年第6卷第34期,共6页

摘　　要：β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K+ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions. 关键词：神经元特异性烯醇化酶脂肪基质细胞细胞分化生理功能成人微管相关蛋白透射电子显微荧光强度

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Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions

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