Cooperation of PprI and DrRRA in response to extreme ionizing radiation in Deinococcus radiodurans

查看全文 作　　者：WANG [1]LiangYan;YIN [2]LongFei;XU [1]GuangZhi;LI [1]MingFeng;ZHANG [1]Hui;TIAN [1]Bin;HUA [1]YueJin

机构地区：[1]Key Laboratory of Ministry of Agriculture for Nuclear-Agricultural Sciences,Institute of Nuclear-Agricultural Sciences,Zhejiang University,Hangzhou 310029,China;[2]Institute of Ecology,Taizhou University,Linhai 317000,China

出　　处：《Chinese Science Bulletin》索引2012年第57卷第1期,共7页

基　　金：supported by Special Fund for Agroscientific Research in the Public Interest(201103007);a major project for Genetically Modified Organisms Breeding(2009ZX08009-075B);a key project from the National Natural Science Foundation of China(30330020);the National Natural Science Foundation of China(31070080);the Fundamental Research Funds for the Central Universities;funded by the China Postdoctoral Science Foundation(20090451464) 摘　　要：Deinococcus radiodurans possesses extreme resistance to ionizing radiation,and has been engineered for the remediation of toxic components in radioactive environments.We have previously shown that PprI(also named IrrE)and DrRRA are essential for the DNA protection and repair pathways that respond to ionizing radiation stress in this species.Here,we investigated the combined roles of PprI and DrRRA in resistance to gamma radiation(800 Gy).The double mutant,△drRRA△pprI,was more sensitive to gamma rays than either△drRRA or△pprI single mutants,and exhibited an elevated level of intracellular protein carbonylation,an extended delay in genome reconstitution and reduced transcriptional levels of certain DNA protection and repair proteins,such as kat,sod,recA and pprA.Interestingly,the induction of DrRRA by ionizing radiation was partially inhibited by the deletion of pprI. Taken together,these results suggest that DrRRA and PprI might have collaborative roles in the response of D.radiodurans to extreme ionizing radiation. 关键词：电离辐射诱导耐辐射球菌公共政策研究所合作超氧化物歧化酶伽玛射线转录水平

相关文献

参考文献（39）
引证文献（7）
耦合文献（14）

参考文献(39)

引证文献(7)

耦合文献(14)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Cooperation of PprI and DrRRA in response to extreme ionizing radiation in Deinococcus radiodurans

参考文献(39)

引证文献(7)

耦合文献(14)

用户提示