Bcl-2shRNA转染联合塞莱昔布对胃癌细胞株SGC-7901的影响(英文)

查看全文 作　　者：Weiwei [1]Liu;Aiqin [1]Wang;Wenjing [1]Xiao;Fangzhen [1]Shen;Minggang [1]Lin;Ruyong [1]Yao

机构地区：[1]Department of Oncology,The Affiliated Hospital of Medical College Qingdao University,Qingdao 266003,China

出　　处：《The Chinese-German Journal of Clinical Oncology》索引2012年第11卷第2期,共5页

摘　　要：Objective:The aim of this study was to study the changes of SGC-7901 cells transfecting small hairpin RNA(shRNA) targeted Bcl-2 and celecoxib in vitro.Methods:To use the effective siRNA targeted to Bcl-2 by Lipofectamine 2000,the rate of cell growth inhibition of all groups was detected by multiply-table tournament(MTT) method,apoptosis rate was examined by flow cytometry and the expression of Bcl-2 was assayed by Elisa.Results:After siRNA was transfected to SGC-7901 cells,the rate of cell growth inhibition was increased,the joint group was higher by flow cytometry and the expression of Bcl-2 was lower by Elisa.Conclusion:The growth of SGC-7901 cells which was transfected siRNA has been inhibited,and the sensitivity to celecoxib has been increased. 关键词：SHRNA BCL-2 细胞转染细胞株 ELISA法 siRNA 生长抑制率流式细胞仪

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Bcl-2shRNA转染联合塞莱昔布对胃癌细胞株SGC-7901的影响(英文)

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