维普中文期刊产品整合服务

Inactivation of the positive LuxR-type oligomycin biosynthesis regulators OlmRI and OlmRII increases avermectin production in Streptomyces avermitilis

查看全文 作　　者：YU [1]Qing;BAI [1]LinQuan;ZHOU [1]XiuFen;DENG [1]ZiXin 高影响力作者 机构地区：[1]State Key Laboratory of Microbial Metabolism,and School of Life Sciences&Biotechnology,Shanghai Jiao Tong University,Shanghai 200030,China高影响力机构 出　　处：《Chinese Science Bulletin》索引2012年第57卷第8期,共8页高影响力期刊 基　　金：supported by National High Technology Research and Development Program of China (2006AA10A202);National Basic Research Program of China (2009CB118901);the National Natural Science Foundation of China (31820015);Ministry of Education of China (706021);the Science and Technology Commission of Shanghai Municipality (075407068) 摘　　要：Oligomycins are a group of 26 macrocyclic lactones that exhibit broad biological activities,including antifungal,anticancer and nematocidal activities.Analysis of the oligomycin biosynthetic gene cluster (olm) in S.avermitilis revealed 2 tandem LuxR-type regulators,OlmRI (931 aa) and OlmRII (941 aa),with shared identity of 38%.Gene replacement of olmRI or olmRII abolished oligomycin production,and this production could be partially restored in the disruptants by introducing cloned olmRI and olmRII with their native promoters,demonstrating the essential role of OlmRI and OlmRII for oligomycin biosynthesis.Quantitative real-time RT-PCR analysis revealed that transcription of 14 olm genes was differentially affected by the deletion of olmRI and olmRII.Unexpectedly,avermectin production in both mutants was enhanced at least 4-fold.The identification of the positive cluster-situated regulators,OlmRI and OlmRII,paves the way for the transcriptional analysis of oligomycin biosynthesis and for the enhancement of oligomycin and avermectin production through regulator engineering. 关 键 词：生物合成基因簇 阿维菌素 生产 监管 R型 链霉菌 RT-PCR分析 灭活
相关文献

参考文献(39)

引证文献(4)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费