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肝癌缺氧诱导因子-lα异常表达与靶向干预的临床价值研究(英文)

查看全文 作　　者：Shanshan [1]Li;Dengfu [2]Yao;Zhizhen [3]Dong;Yajie [4]Qian;Dandan [1]Yu;Ninghua [1]Yao;Jie [2]Chen;Xiaodi [2]Yan;Chenglin [2]Qin 高影响力作者 机构地区：[1]Oncology Center, Affiliated Hospital of Nantong University, Nantong 226001, China;[2]Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China;[3]Department of Diagnostics, Affiliated Hospital of Nantong University, Nantong 226001, China;[4]Medical College of Nantong University, Nantong 226001, China高影响力机构 出　　处：《The Chinese-German Journal of Clinical Oncology》索引2012年第11卷第3期,共5页高影响力期刊 基　　金：Supported by grants from Jiang su Health Key Project(No.K201102);Nantong City Social Development Project (No. S2009027) 摘　　要：Objective:The aim of this study was to analyze the expression features of hypoxia inducible factor-1α (HIF-1α) in hepatocellular carcinoma (HCC) and effects of HIF-1α silencing on HepG2 cells.Methods:HIF-1α expression was analyzed in the self-control HCC specimens by immunohistochemistry.After HepG2 cells with miRNA transfection,the expression of HIF-1α was determined at mRNA or protein level by real-time polymerase chain reaction (PCR) or Western blotting.Vascular endothelial growth factor (VEGF) and angiopoietin-2 (ANG-2) were determined by ELISA.Alterations of cell cycles and apoptosis of HepG2 cells were measured using a flow cytometer.Results:Positive HIF-1α was brown and granule-like in the cytoplasm or nucleus.Significant difference was found between HCC (80%) and its surrounding tissues (100%,χ2=22.35,P < 0.001) and HIF-1α expression related to tumor size.At 72 h after miRNA transfection,the expression of HIF-1α in HepG2 cells was down-regulated by 87% at mRNA or 65% at protein level,with VEGF and ANG-2 decreased to 54% and 36%,respectively.After RNA interference combined with anti-cancer drug,the apoptotic rate of HepG2 cells was increasing from 22.46% ± 0.61% to 36.99% ± 0.88%,with up-regulation of G1 phase (65.68% ± 0.91%) and down-regulation of S phase (19.47 ± 1.34 %).Conclusion:Abnormal expression of HIF-1α is associated with development of HCC,and HIF-1α gene silencing can effectively inhibit HepG2 cell proliferation. 关 键 词：缺氧诱导因子1 肝癌细胞 RNA干扰 临床价值 HEPG2细胞 异常 HIF-1 血管内皮生长因子
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