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Tumor necrosis factor-alpha -308G/A polymorphism and risk of hepatocellular carcinoma in hepatitis C virus-infected patients

查看全文 作　　者：Roba [1]M.Talaat;Ahmed [2]A.Esmail;Reda [3]Elwakil;Adel [1]A.Gurgis;Mahmoud [1]I.Nasr 高影响力作者 机构地区：[1]Molecular Biology Departments,Genetic Engineering and Biotechnology Research Institute(GEBRI),Menofia University,Sadat 22857,Egypt;[2]Roche Diagnostics,Ain Shams University,Cairo 11522,Egypt;[3]Department of Tropical Medicine,Ain Shams University,Cairo 11522,Egypt高影响力机构 出　　处：《Chinese Journal of Cancer》索引2012年第31卷第1期,共7页高影响力期刊 摘　　要：Tumor necrosis factor-alpha (TNF-α) is an important cytokine in generating an immune response against infection with hepatitis C virus (HCV). The functions of TNF-α may be altered by single-nucleotide polymorphisms (SNPs) in its gene structure. We hypothesized that SNPs in TNF-α may be important in determining the outcome of an HCV infection. To test this hypothesis, we investigated the role of the polymorphism -308G/A, which is located in the promoter region of the TNF-α gene, in the progression of HCV infection in Egyptian patients using a quantitative real-time polymerase chain reaction (qRT-PCR). The distribution of this polymorphism and its impact on the serum level of TNF-α was compared between 90 HCV-infected patients [45 with HCV-induced cirrhosis and 45 with HCV-related hepatocellular carcinoma (HCC)] and 45 healthy Egyptian volunteers without any history of liver disease. Our results showed that at the TNF-α -308 position, the G/G allele was most common (78.5% ) in the study population, with the G/A and A/A alleles occurring less frequently (13.3% and 8.1% , respectively). Frequencies of G/G, G/A, and A/A genotypes were 87%, 7%, and 6% in patients with liver cirrhosis and were 94% , 4% , and 2% in patients with HCC, respectively. Serum levels of TNF-α were significantly higher in HCV-infected patients than in healthy controls, indicating that the TNF-α -308 polymorphism does not influence the production of TNF-α. The serum level of TNF-α was positively correlated with HCV infection. Taken together, these findings suggest that the TNF-α -308 polymorphism may not be a host genetic factor associated with the severity of HCV infection, but may be an independent risk factor for HCC. 关 键 词：肿瘤坏死因子-Α 单核苷酸多态性 丙型肝炎病毒 病毒感染 患者 肝癌 风险 聚合酶链反应
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