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Resveratrol inhibits angiotensin II-induced ERK1/2 activation by downregulating quinone reductase 2 in rat vascular smooth muscle cells

查看全文 作　　者：Xiwen [1]Zhang;Yao [1]Wang;Weiwei [2]Yang;Xiaofeng [1]Hou;Jiangang [1]Zou;Kejiang [1]Cao 高影响力作者 机构地区：[1]Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China;[2]Department of Nephrology, Huai'an First People 's Hospital affiliated to Nanjing Medical University, Huai'an, China高影响力机构 出　　处：《The Journal of Biomedical Research》索引2012年第26卷第2期,共7页高影响力期刊 基　　金：supported by grants from the National Natural Science Foundation of China (No.30971255) 摘　　要：Our previous studies showed that resveratrol could inhibit the proliferation of vascular smooth muscle cells (VSMCs) and repress mRNA and protein expression of quinone reductase 2 (NQO2).This study further explored the potential mechanisms whereby resveratrol inhibits the proliferation of rat VSMCs.Lentiviral vectors that incorporated NQO2 small interfering RNA (siRNA) were constructed and transduced into rat VSMCs.The cell proliferation was detected using the bromodeoxyuridine (BrdU) assay.Cultured rat VSMCs were stimulated with angiotensin II and the level of reactive oxygen species (ROS) was measured using a ROS assay kit.A realtime quantitative PCR was used to detect NQO2 mRNA levels.Extracellular signal-regulated kinase (ERK1/2) and NQO2 protein expression were determined by Western blotting analysis.The inhibitory effect of resveratrol (10 and 50 μmol/L) on the proliferation of rat VSMCs in the NQO2 siRNA group was significantly weaker than that in the normal and scrambled siRNA group (P < 0.01).The ROS level in the NQO2 siRNA and resveratrol (50 μmol/L) treatment groups were lower than that in the normal and scrambled siRNA groups (P < 0.01 in both).Compared with the normal and scrambled siRNA group,the phosphorylation of ERK1/2 was significantly decreased in the NQO2 siRNA and resveratrol (50 μmol/L) treatment group (P < 0.01 in both).In conclusion,high concentration of resveratrol inhibits angiotensin II-induced ERK1/2 phosphorylation and subsequent proliferation by down-regulation of NQO2 in cultured rat VSMCs. 关 键 词：血管平滑肌细胞 血管紧张素Ⅱ 白藜芦醇 还原酶 大鼠 诱导 MRNA水平 细胞外信号调节激酶
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