Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

查看全文 作　　者：Kang [1,2]Kang;Xiao [1]Peng;Jun [2]Luo;Deming [1]Gou

机构地区：[1]College of Life Science,Shenzhen University,Shenzhen,Guangdong,518060,China;[2]College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,712100,China

出　　处：《Journal of Animal Science and Biotechnology》索引2012年第3卷第2期,共9页

基　　金：supported by National Natural Science Fundation of China(grant No. 81170047);Science Industry Trade and Information Technology Commission of Shenzhen Municipality, China (grant No.JC201006010725A) 摘　　要：MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs in serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction (PCR)-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps: (1) sample collection and preparation; (2) global miRNAs profiling using quantitative real-time PCR (qRT-PCR); (3) data normalization and analysis; and (4) selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers. 关键词：MIRNAS 实时定量PCR 生物标志物血液循环技术分析基础心血管疾病识别

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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

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耦合文献(146)

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