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Increased frequency and clinical significance of myeloidderived suppressor cells in human colorectal carcinoma

查看全文 作　　者：Hong-Li [1]Sun,;Xin [1]Zhou,;Yi-Feng [1,4]Xue;Ke [2]Wang,;Yun-Feng [1]ShenI;Jing-Jue [1]Mao;Hong-Feng [1]Guo;Zong-Ning [3]Miao 高影响力作者 机构地区：[1]Department of Hematology, Wuxi People'sHospital,Wuxi 214023, Jiang,Provlnce, China;[2]Department of General Surgery, Wuxi Third People'sHospital.Wuxi 214023, Jiangsu Province, China;[3]The Stem Cell Research Laboratory, WuxiThird People's Hospital, Wuxi 214023, Jiangsu Province, China;[4]Department of Clinical Laboratory, Wuxi People'sHospital, Wuxi 214023, Jiangsu Province, China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2012年第18卷第25期,共7页高影响力期刊 摘　　要：AIM:To investigate the frequency and clinical significance of the myeloid-derived suppressor cells(MDSC) in human colorectal carcinoma(CRC).METHODS:Samples of peripheral blood and tumor tissue from 49 CRC patients were analyzed.Mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation and were subjected to a flow cytometrybased immunophenotypic analysis.RESULTS:A considerable increase in the percentage of CD33+HLA-DR-MDSCs was observed in the peripheral blood(1.89% ± 0.75%) and tumor tissues(2.99% ± 1.29%) of CRC patients as compared with that in theperipheral blood of healthy controls(0.54% ± 0.35%).This expanded CD33+HLA-DR-subset exhibited immature myeloid cell markers,but not lineage markers,and showed up-regulation of CD18/CD11b expression as compared with the MDSCs from healthy donors.Further studies showed that the MDSC proportion in CRC peripheral blood was correlated with nodal metastasis(P = 0.023),whereas that in tumor tissues was correlated with nodal/distant metastasis(P = 0.016/P = 0.047) and tumor stage(P = 0.028),suggesting the involvement of MDSCs in CRC tumor development.CONCLUSION:Characterization of MDSCs in CRC suggests the clinical significance of circulating and tumorinfiltrating MDSCs and may provide new insights into the CRC immunotherapy targeting MDSCs. 关 键 词：单核细胞 临床意义 人类 频率 大肠癌 密度梯度离心法 肿瘤组织 结直肠癌
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