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Enhanced oxidation of benzo[a]pyrene by crude enzyme extracts produced during interspecific fungal interaction of Trametes versicolor and Phanerochaete chrysosporium

查看全文 作　　者：Linbo [1,2]Qian Baoliang [1,2]Chen 高影响力作者 机构地区：[1]Department of Environmental Science, Zhejiang University, Hangzhou 310058, China.;[2]Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China高影响力机构 出　　处：《Journal of Environmental Sciences》索引2012年第24卷第9期,共8页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(No.41071210,20890111);the National High-Tech Research and Development(863) Program of China(No.2012AA06A203);the Provincial Natural Science Foundation of Zhejiang(No.R5100105);the Doctoral Fund of the Ministry of Education of China(No.J20091588) 摘　　要：The effects of interspecific fungal interactions between Trametes versicolor and Phanerochaete chrysosporium on laccase activity and enzymatic oxidation of polycyclic aromatic hydrocarbons(PAHs) were investigated.A deadlock between the two mycelia rather than replacement of one fungus by another was observed on an agar medium.The laccase activity in crude enzyme extracts from interaction zones reached a maximum after a 5-day incubation,which was significantly higher than that from regions of T.versicolor or P.chrysosporium alone.The enhanced induction of laccase activity lasted longer in half nutrition than in normal nutrition.A higher potential to oxidize benzo[a]pyrene by a crude enzyme preparation extracted from the interaction zones was demonstrated.After a 48 hr incubation period,the oxidation of benzo[a]pyrene by crude enzyme extracts from interaction zones reached 26.2%,while only 9.5% of benzo[a]pyrene was oxidized by crude extracts from T.versicolor.The oxidation was promoted by the co-oxidant 2,2'-azinobis-3ethylbenzthiazoline-6-sulphonate diammonium salt(ABTS).These findings indicate that the application of co-culturing of white-rot fungi in bioremediation is a potential ameliorating technique for the restoration of PAH-contaminated soil. 关 键 词：苯并[A]芘 酶促氧化 相互作用 白腐真菌 提取物 白腐菌 漆酶活性 PAHs
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