A Widely Applicable Method for Plant Genomic DNA Extraction

查看全文 作　　者：Bin [1]LI;Lixia [1]YU;Junjun [1]XI;Yaping [1]ZHANG;Zerui [2]GAO;Bo [1]YAN

机构地区：[1]Southwest Forestry University;[2]Yunnan Academy of Tobacco Science

出　　处：《Agricultural Biotechnology》索引2012年第1卷第1期,共2页

基　　金：Supported by National Natural Science Foundation of China(31060042);Applied Basic Research Project of Yunnan Province(2009ZC079M) 摘　　要：[Objective] This study aimed to explore a method for plant genomic DNA extraction,to provide guidance for rapid extraction of genomic DNA from plant tissues.[Method] Based on the published genomic DNA extraction methods,operation steps,reagent amount and processing time of SDS extraction method were optimized.[Result] A widely applicable method was established initially for genomic DNA extraction from various varieties of plants and various kinds of plant tissues.Quality of the extracted genomic DNA was relatively good,which meets the requirements for further operation.[Conclusion] This study provided guidance for rapid plant genomic DNA extraction. 关键词：基因组DNA提取植物组织提取方法操作步骤快速提取试剂用量 SDS

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A Widely Applicable Method for Plant Genomic DNA Extraction

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