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Construction of DNA Fingerprint Using SSR Marker for Hybrid Rice Cultivars Approved by Hunan Province

查看全文 作  者:Qingcai [1,2]ZHAN;Zifa [1,2]XIAO;Keyong [1]ZHU;Zhixi [1]LIU;Zhaohua [1]LI;Shuzhen [1]ZENG 高影响力作者 机构地区:[1]Laboratory of Biotechnology,Hunan Rice Research Institute;[2]Graduate School of Central South University,Longping Branch高影响力机构 出  处:《Agricultural Biotechnology》索引2012年第1卷第4期,共5页高影响力期刊 基  金:Supported by Agricultural Science and Technology Achievements Transformation Fund of Ministry of Science and Technology (2007GB2D200225);Master Degree Paper Innovation Fund of Central South University (2010SSXt045) 摘  要:[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify different hybrid rice cultivars. [Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification. 关 键 词:指纹图谱构建 DNA指纹 水稻品种 杂交水稻 湖南省 SSR标记 SSR引物 DNA分子
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