Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

查看全文 作　　者：NIU Bu-[1,2]yue;LAN Xiao-[1]ming;YAN Xiao-[1]hong;DI Sheng-[1]wei;WANG [1]Yang;LI Hai-[1]tao;XIONG Yuan- [2]zhu;WANG Xi-[1]biao

机构地区：[1]College of Animal Science and Technology, Northeast Agricultural University;[2]Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/Huazhong Agricultural University

出　　处：《Journal of Integrative Agriculture》索引2013年第12卷第1期,共7页

基　　金：supported by the Educational Commission of Heilongjiang Province, China (11541031) 摘　　要：The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS). 关键词：基质金属蛋白酶产仔数性状候选基因长白猪分子标记辅助选择 GENBANK 单核苷酸多态性表征

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Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

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