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Comparative study of the promotion of porcine fetal fibroblast proliferation by overexpression of two transcriptional variants of SIRT6

查看全文 作　　者：XIE [1,2]XiaoXian;HE [1]Kan;ZHANG [2,3]AiLing;ZHANG [2,3]Hao;WANG [1]QiShan;LI [2,3]JiaQi;MA [1]YuFang 高影响力作者 机构地区：[1]School of Agriculture and Biology,Department of Animal Sciences,Shanghai Jiao Tong University,Shanghai Key Laboratory of Veterinary Biotechnology;[2]Department of Animal Genetics,Breeding and Reproduction,College of Animal Sciences,South China Agricultural University;[3]Guangdong Provincial Key Laboratory of Agroanimal Genomics and Molecular Breeding,College of Animal Sciences,South China Agricultural University高影响力机构 出　　处：《Chinese Science Bulletin》索引2013年第58卷第10期,共6页高影响力期刊 基　　金：supported by the National Special Fundation for Transgenic Species of China (2011ZX08006-004);the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-36) 摘　　要：Mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and rDNA.However,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and development.In this study,a novel variant of porcine SIRT6(SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA(SIRT6 V1).Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts(PFFs) were compared.Cells transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2(P<0.01),though both proliferated significantly faster than cells expressing an empty vector(P<0.01).These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 V1.These results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF proliferation.Deletion of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation,possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure. 关 键 词：成纤维细胞增殖 胎儿成纤维细胞 DNA转录 过度表达 猪 变体 聚合酶链反应 BLAST
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