The complete mitochondrial genome of the grey bamboo shark (Chiloscyllium griseum) (Orectolobiformes: Hemiscylliidae): genomic characterization and phylogenetic application

查看全文 作　　者：CHEN [1,2]Xiao;AI [3]Weiming;YE [4]Le;WANG [4]Xuehui;LIN [2]Chongwen;YANG [5]Shengyun 机构地区：[1]College of Fisheries, Ocean University of China;[2]Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute;[3]Department of Marine Science, School of Life Science, Wenzhou Medical College;[4]South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;[5]Laboratory of Marine Biodiversity and Global Change, College of Ocean and Earth Sciences, Xiamen University 出　　处：《Acta Oceanologica Sinica》索引2013年第32卷第4期,共7页 基　　金：The National Natural Science Foundation of China under contract No.41006080;Ocean Public Welfare Scientific Research Project under contract Nos 200905019 and 201105008-4;Science and Technology Department of Zhejiang Province,China under contract No.2007F10011 摘　　要：The complete mitochondrial genome of the grey bamboo shark, Chiloscyllium griseum (Orectolobiformes: Hemiscylliidae) was obtained, with a total of 16 755 base pairs (bp). The mitogenome encoded 13 proteincoding genes, two ribosomal RNAs, 22 transfer RNAs and a noncoding control region, a gene arrangement identical to the most common type found in vertebrates. All tRNA sequences were folded into typical cloverleaf secondary structure with the exception of tRNA-Ser2, in which the DHU arm stem was replaced with 12 unpaired nucleotides and formed a simple loop. In the control region, the putative termination-associated sequences (TAS) with hairpin-loop structure and the conserved sequence blocks (CSB) Ⅰ-Ⅲ were identified and considered to be associated with the replication and transcription of mtDNA. No repeat motifs were found in the mitogenome of C. griseum. On the basis of the mitogenomic data available in the Class Chondrichthyes from both Genbank and this study, and the phylogenetic analyses using maximum likelihood (ML) and Bayesian inference (BI) methods, the results strongly support the basal division between batoids and sharks and the monophyly of the Superorder Galeomorphii, and confirm the phylogenetic position of C. griseum in the Subclass Elasmobranchii. This study suggests that the mitogenomic data are more robust for reconstructing phylogenetic relationships than individual genes in the Elasmobranchii. 关 键 词：系统发育分析 线粒体基因组 基因组特性 斑竹 鲨鱼 灰色 GENBANK RNA序列