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Interleukin-12 as a Genetic Adjuvant Enhances Hepatitis C Virus NS3 DNA Vaccine Immunogenicity

查看全文 作　　者：Malihe [1]Naderi;Atefeh [2]Saeedi;Abdolvahab [3]Moradi;Mishar [3]Kleshadi;Mohammad Reza [1]Zolfaghari;Ali [4,5,6]Gorji;Amir [3,4]Ghaemi 高影响力作者 机构地区：[1]Department of Microbiology, Qom branch, Islamic Azad University;[2]Department of Microbiology, Lahijan Branch, Islamic Azad University;[3]Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Department of Microbiology, Golestan University of Medical Sciences;[4]Shefa Neuroscience Research Centre;[5]Institut für Physiologie I, Westflische Wilhelms-Universitt Münster;[6]Klinik und Poliklinik für Neurochirurgie, Westf lische Wilhelms-Universitt Münster高影响力机构 出　　处：《Virologica Sinica》索引2013年第28卷第3期,共7页高影响力期刊 摘　　要：Hepatitis C virus (HCV) chronic infection is a worldwide health problem, and numerous efforts have been invested to develop novel vaccines. An efficient vaccine requires broad immune response induction against viral proteins. To achieve this goal, we constructed a DNA vaccine expressing nonstructural 3 (NS3) gene (pcDNA3.1-HCV-NS3) and assessed the immune response in C57BL/6 mice. In this study, the NS3 gene was amplified with a nested-reverse transcriptase-polymerase chain reaction (RT-PCR) method using sera of HCV-infected patients with genotype 1a. The resulting NS3 gene was subcloned into a pcDNA3.1 eukaryotic expression vector, and gene expression was detected by western blot. The resultant DNA vaccine was co-administered with interleukin-12 (IL-12) as an adjuvant to female C57BL/6 mice. After the final immunizations, lymphocyte proliferation, cytotoxicity, and cytokine levels were assessed to measure immune responses. Our data suggest that co-administration of HCV NS3 DNA vaccine with IL-12 induces production of significant levels of both IL-4 and interferon (IFN)-γ (p<0.05). Cytotoxicity and lymphocyte proliferation responses of vaccinated mice were significantly increased compared to control (p<0.05). Collectively, our results demonstrated that co-administration of HCV NS3 and IL-12 displayed strong immunogenicity in a murine model. 关 键 词：丙型肝炎病毒 DNA疫苗 白细胞介素-12 免疫原性 佐剂 淋巴细胞增殖 IL-12 白细胞介素12
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