Mining of a streptothricin gene cluster from Streptomyces sp.TP-A0356 genome via heterologous expression

查看全文 作　　者：LI [1]JinE;GUO [1]ZhengYan;HUANG [2]Wei;MENG [1,3]XiangXi;AI [1]GuoMin;TANG [2]GongLi;CHEN [1]YiHua

机构地区：[1]State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences;[2]State Key Laboratory of Bio-organic and Natural Product Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences;[3]University of Chinese Academy of Sciences

出　　处：《Science China(Life Sciences)》索引2013年第56卷第7期,共9页

基　　金：supported in part by the National Natural Science Foundation of China (31170037);Ministry of Science and Technology of China (2013CB734003);the China Postdoctoral Science Foundation (2013M530755) 摘　　要：Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying -lysines for the poly-β-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence. 关键词：天蓝色链霉菌基因组测序异源表达基因簇矿业生物合成途径聚赖氨酸实验证据

相关文献

参考文献（39）
引证文献（4）
耦合文献（0）

参考文献(39)

引证文献(4)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Mining of a streptothricin gene cluster from Streptomyces sp.TP-A0356 genome via heterologous expression

参考文献(39)

引证文献(4)

用户提示