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Effect of Kushen(Radix Sophorae flavescentis) extract on laryngeal neoplasm Hep2 cells

查看全文 作　　者：Yingzi [1]Wang;Chunchao [2]Han;Xinsheng [3]Fang;Xinyuan [1]Shi;Ailing [1]Feng;Kuibang [1]He;Shenghai [1]Zhang;Xiuyu [1]Sun 高影响力作者 机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine;[2]School of Pharmacy,Shandong University of Traditional Chinese Medicine;[3]College of Agronomy,Shandong Agricultural University高影响力机构 出　　处：《Journal of Traditional Chinese Medicine》索引2013年第33卷第2期,共5页高影响力期刊 基　　金：Supported by the National Natural Science Foundation Project of China (No. 81274082);the Research Project for Nonprofit Industry of State Administration of TCM (No.201007012-2-11) 摘　　要：OBJECTIVE:To evaluate the effect of fermented extract of Kushen(Radix Sophorae Flavescentis) or non-fermented ESF on laryngeal neoplasms Hep2 cells.METHODS:Use 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells,and detect the mRNA and protein expression level of Bcl-2,Bax and Caspase-3 with reverse transcription polymerase chain reaction(RT-PCR) andWestern blot.RESULTS:Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm's Hep2 cells,but and the cells did not response to the dilution 1:320 of fermented ESF,nor to the 1:1280 dilution of non-fermented ESF.As time progressed,the dilution 1:80 of fermented ESF and 1:320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression.Bax mRNA and protein were not expressed in Hep2 cells.CONCLUSIONS:Both fermented ESF and non-fermented ESF could inhibit the proliferation of Hep2 cells,and the effect of non-fermented ESF was significantly better than that of the fermented. 关 键 词：苦参碱 细胞 Caspase-3 肿瘤 喉部 反转录聚合酶链反应 mRNA 蛋白表达
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