Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells

查看全文 作　　者：Xiuling [1]Yu;Dianwen [1]Gao

机构地区：[1]Department of Ophthalmology,Shengjing Hospital,China Medical University

出　　处：《Neural Regeneration Research》索引2013年第8卷第23期,共6页

摘　　要：A plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1α in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible fac-tor-1α expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increas-ing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-1α are strongly up-regulated by hypoxia, and that there is a strong relationship between hy-poxia-inducible factor-1α and cytoglobin during hypoxic injury. 关键词：SH-SY5Y细胞缺氧诱导因子神经母细胞瘤蛋白基因过度表达损伤 WESTERN印迹质粒转染

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Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells

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