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Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.)

查看全文 作  者:Touming [1]Liu;Haiyang [1]Liu;Huang [1]Zhang;Yongzhong [1]Xing 高影响力作者 机构地区:[1]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University高影响力机构 出  处:《Journal of Integrative Plant Biology》索引2013年第55卷第10期,共11页高影响力期刊 基  金:supported by grants from the National Natural Science Foundation of China(30921091);the National Key Program on Basic Research and Development(2010CB125901);the National Special Program for Research of Transgenic Plants of China(2011ZX08009‐001‐002);the Fundamental Research Funds for the Central Universities(2012YB03) 摘  要:A quantitative trait locus(QTL)that affects heading date(HD)and the number of spikelets per panicle(SPP)was previously identifed in a small region on chromosome 7 in rice(Oryza sativa L.).In order to further characterize the QTL region,near isogenic lines(NILs)were quickly obtained by self‐crossing recombinant inbred line 189,which is heterozygous in the vicinity of the target region.The pleiotropic effects of QTL Ghd7.1 on plant height(PH),SPP,and HD,were validated using an NIL‐F2 population.Ghd7.1 explained 50.2%,45.3%,and 76.9% of phenotypic variation in PH,SPP,and HD,respectively.Ghd7.1 was precisely mapped to a 357‐kb region on the basis of analysis of the progeny of the NIL‐F2 population.Day‐length treatment confrmed that Ghd7.1 is sensitive to photoperiod,with long days delaying heading up to 12.5 d.Identifcation of panicle initiation and development for the pair of NILs showed that Ghd7.1 elongated the photoperiod‐sensitive phase more than 10 d,but did not change the basic vegetative phase and the reproductive growth phase.These fndings indicated that Ghd7.1 regulates SPP by controlling the rate of panicle differentiation rather than the duration of panicle development. 关 键 词:数量性状基因座 ORYZA 多效性 抽穗期 水稻 遗传位点 验证 表征
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