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Biological Characteristics of Fusarium oxysporum f. sp.niveum Isolated from Watermelon Cultivated in Plastic Greenhouse and Indoor Toxicity Determination of Different Fungicides in Hainan Province of China

查看全文 作  者:Fan [1,2]Hongyan;Zhou [1,2]Wenjing;Wang [1,2]Xianghe;Luo [1,2]Zhiwen;He [1,2]Fan 高影响力作者 机构地区:[1]Institute of Tropical Fruit Tree,Hainan Academy of Agricultural Science;[2]Haikou Investigation Station of Tropical Fruit Trees,Ministry of Agriculture高影响力机构 出  处:《Plant Diseases and Pests》索引2013年第4卷第5期,共6页高影响力期刊 基  金:Supported by Epidemic Monitoring and Control Program of Pests and Diseasesin Tropical Crops in 2013 by Ministry of Agriculture(Issued Document Number:NBK[2013]No.28) 摘  要:[Objective]The paper was to study biological characteristics of Fusarium oxysporum f.sp.niveum in watermelon cultivated in plastic greenhouse in Hainan Province of China and to determine indoor toxicity of fungicides.[Method]Effects of various factors on mycelial growth and spore germination were studied,and the lethal temperature of spores and indoor toxicity of fungicides were also determined.[Result]The fungal mycelium had a better growth rate on watermelon juice medium than the others.The optimum temperature for mycelial growth and spore germination were 28℃and 30℃,and the optimum pH values were7-8 and 7-9,respectively.Light-dark alternation was conducive for mycelial growth.The lethal temperature of spore was 60℃for 5 min.The fungal mycelium grew better while taking fructose and glucose as carbon sources and yeast extract and peptone as nitrogen sources.Toxicity determination showed that 50%prochloraz-manganese chloride complex WP had the greatest inhibition effect on mycelial growth,with the EC50value of 0.730 9μg/mL;followed by 32.5%benzoic azoxystrobin SC,30%benzoylate·propiconazol EC,10%difenoconazole WG,25%bromothalonil·carbendazim WP and 25%bromothalonil WP,with the EC50values from 1.884 7μg/mL to 8.161 0μg/mL.[Conclusion]The study provided basic data for field control against F.oxysporum f.sp.niveum on watermelon cultivated in plastic greenhouse in Hainan Province. 关 键 词:室内毒力测定 生物学特性 塑料大棚 西瓜汁 海南省 杀菌剂 SP 多菌灵可湿性粉剂
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