Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

查看全文 作　　者：Shi-Ping [1]Liu;Jia-Xin [1]Yang;Dong-Yan [1]Cao;Keng [1]Shen

机构地区：[1]Department of Obstetrics and Gynecology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences

出　　处：《Cancer Biology & Medicine》索引2013年第10卷第3期,共4页

摘　　要：Objective:To identify differentially expressed long non-coding RNAs(lncRNAs) involved in the metastasis of epithelial ovarian cancer.Methods:An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and SKOV3.ip1 cell lines.Total RNA was then extracted,and microarray analysis was performed.Moreover,nine lncRNAs were selected for validation using RT-qPCR.Results:Compared with the SKOV3 cells,the SKOV3.ip1 cells significantly improved in the in vitro invasive activity.Of the 4,956 lncRNAs detected in the microarray,583 and 578 lncRNAs were upregulated and downregulated,respectively,in SKOV3.ip1 cells,compared with the parental SKOV3 cells.Seven of the analyzed lncRNAs(MALAT1,H19,UCA1,CCAT1,LOC645249,LOC100128881,and LOC100292680) confirmed the deregulation found by microarray analysis.Conclusion:LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials.This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis.Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis. 关键词：卵巢癌细胞非编码RNA 差异表达微阵列分析鉴定人类 RNAS 放松管制

相关文献

参考文献（27）
引证文献（28）
耦合文献（0）

参考文献(27)

引证文献(28)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

参考文献(27)

引证文献(28)

用户提示