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Changes of Rubisco in Flag Leaves of Wheat with Different Quality Types during Grain Filling Stage

查看全文 作　　者：Peng [1]ZHANG;Jinbao [1]YAO;Guocai [1]YAO;Xueming [1]YANG;Hongxiang [1]MA 高影响力作者 机构地区：[1]Provincial Key Lab for Agrobiology,Jiangsu Academy of Agricultural Sciences高影响力机构 出　　处：《Agricultural Biotechnology》索引2013年第2卷第6期,共4页高影响力期刊 基　　金：Supported by Science and Technology Support Program of Jiangsu Province(BE2012305);Natural Science Foundation of Jiangsu Province(BK2011670);'Twelfth Five-Year' National Science and Technology Support Program of China(2011BAD35B03) 摘　　要：[Objective]In this study,the formation mechanism of grain quality of soft wheat was investigated to provide support for soft wheat breeding.[Method]Using soft and hard wheat varieties as experimental materials,changes of Rubisco(ribulose-1,5-bisphosphate carboxylase/oxygenase)degradation in flag leaves with different quality types during grain filling stage were investigated.[Result]Chlorophyll content and Rubisco content were both gradually reduced in flag leaves of wheat cultivars with different quality types during grain filling stage.Changes of Rubisco content were insignificant at early grain filling stage,but Rubisco content in flag leaves of soft wheat was higher than that of hard wheat at mid-late grain filling stage.Degradation products of Rubisco large subunits with molecular weight of 51 kD could be detected in flag leaves of various wheat cultivars.Rubisco in flag leaves of hard wheat Zheng 9023 was significantly earlier degraded compared with Yangmai 15,indicating that leaf senescence in hard wheat was earlier than soft wheat,which suggested that the protein accumulation in wheat grains may be related with Rubisco degradation in flag leaves after anthesis.[Conclusion]This study implied the foundation of soft wheat breeding and optimization of cultivation and wheat management in the field. 关 键 词：Rubisco含量 籽粒灌浆期 小麦旗叶 品质类型 加氧酶 小麦品种 二磷酸羧化酶 降解产物
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