The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells

查看全文 作　　者：Jielin [1]Zhang;Yuqiu [1]Mao;Li [2]Hou;Xiaonan [1]Cui

机构地区：[1]Department of Oncology,The First Affiliated Hospital of Dalian Medical University;[2]Department of Pathology,Dalian Medical University

出　　处：《Chinese Journal of Cancer Research》索引2013年第25卷第6期,共7页

基　　金：The General Program of National Natural Science Foundation of China:Research Fund for the mechanism of Arenobufagin space isomer inhibits lymphatic metastasis of mouse hepatocarcinoma;The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry;Specialized Research Fund for the Doctoral Program of Higher Education(NO.20102105120002) 摘　　要：Objective:To investigate the impact of beta-elemene injection on the growth and alpha-tubule of human hepatocarcinoma HepG2 cells.Methods:Cell proliferation was assessed by MTT assay.Cell cycle distribution was detected by flow cytometry(FCM).The mRNA expression of alpha-tubulin was measured by RT-PCR.Western blot analysis was used to determine protein expression of alpha-tubulin and the polymerization of tubulin.Results:Beta-elemene injection inhibited HepG2 cells proliferation in a dose-and time-dependent manner;FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase.RT-PCR and westernblot analysis showed that beta-elemene injection down-regulated alpha-tublin at both mRNA and protein levels,presenting a dose-dependent manner.Moreover,beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner.Conclusions:Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells and induce cell apoptosis,the mechanism might be partly related to the down-regulation of alpha-tubulin and inhibition of microtubular polymerization. 关键词：HepG2细胞 Α-微管蛋白 Β-榄香烯聚合 RT-PCR检测肝癌 WESTERN印迹时间依赖性

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The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells

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