Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry

查看全文 作　　者：ZHANG [1,2,3]YanDong;LI [1,2,3]HaiFang;MA [1,2,3]Yuan;LIN [1,2,3]JinMing

机构地区：[1]Beijing Key Laboratory of Microanalytical Methods and Instrumentation;[2]The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology;[3]Department of Chemistry, Tsinghua University

出　　处：《Science China Chemistry》索引2014年第57卷第3期,共5页

基　　金：supported by the National Natural Science Foundation of China(21227006,21275088) 摘　　要：The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process.In this work,a mass spectrometry-based method for direct,in situ analysis of lipids in cells was reported.Mammalian cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry(MALDI-MS).The matrix application process was achieved by electrospray coating,which produced a homogenous layer of matrix crystal on the sample.The detection results and reproducibility are satisfactory.With this method,a profile of abundant membrane lipids is generated,which is characteristic of cell type.In conclusion,this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells. 关键词：基质辅助激光解吸电离质谱哺乳动物细胞原位分析 MALDI-MS 分子分析膜脂质心理过程 ITO膜

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Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry

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