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Rapid and sensitive determination of fatty acids in edible oil by liquid chromatography-electrospray ionization tandem mass spectrometry

查看全文 作　　者：BI [1]Xin;JIN [1]YiBao;LI [2]ShangFu;GAO [3,4]Dan;JIANG [3,4]YuYang;LIU [3,4]HongXia 高影响力作者 机构地区：[1]Department of Chemistry, Tsinghua University;[2]Department of Chemistry, Hong Kong Baptist University;[3]State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen,Tsinghua University;[4]Key Laboratory of Metabolomics at Shenzhen高影响力机构 出　　处：《Science China Chemistry》索引2014年第57卷第3期,共6页高影响力期刊 基　　金：grateful to Ministry of Science and Technology of China under Contract(2012ZX09506001-010);the National High Technology Research and Development Program of China(2013AA092902);the National Natural Science Foundation of China(21172129)for financial support 摘　　要：A sensitive and robust on-line LC/MS method was developed for quantitative determination of linoleic acid,docosahexaenoic acid and docosanoic acid from edible oil samples.The oil samples were dissolved in chloroform-isopropyl alcohol(20:80,v:v)solution and the three fatty acids were separated by HPLC with a C4 column using 10 mmol/L ammonium acetate-isopropyl alcohol-acetonitrile(20:40:40,v:v:v)mobile phase in isocratic elution.Electrospray ionization mass spectrometry with the selected ion recording monitoring was used to detect and quantify the fatty acid.The calibration curves were linear in the range of 10.00–5000 pg/mL for linoleic acid and docosanoic acid,and 1.000–500.0 pg/mL for docosahexaenoic acid.The limit of detection was 2.0 pg/mL for linoleic acid,3.0 pg/mL for docosanoic acid,and 0.20 pg/mL for docosahexaenoic acid.The results showed that the method described in this paper could be utilized for rapid determination of three fatty acids at picogram levels in edible oils. 关 键 词：高效液相色谱法 电喷雾电离质谱 定量测定 脂肪酸 食用油 串联质谱法 灵敏 色谱 质谱法
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