Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil

查看全文 作　　者：Evi [1,2]Kurniati;Novi [3]Arfarita;Tsuyoshi [2]Imai;Takaya [2]Higuchi;Ariyo [2]Kanno;Koichi [2]Yamamoto;Masahiko [2]Sekine

机构地区：[1]Department of Agricultural Engineering, Brawijaya University;[2]Division of Environmental Science and Sustainable Engineering, Graduate School of Science and Engineering, Yamaguchi University;[3]Department of Agrotechnology, Malang Islamic University

出　　处：《Journal of Environmental Sciences》索引2014年第26卷第6期,共9页

基　　金：High gratitude to Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia for the full support through DIKTI Scholarship, Brawijaya University, and Yamaguchi University 摘　　要：The use of filamentous fungi in bioremediation of heavy metal contamination has been developed recently. This research aims to observe the capability of filamentous fungi isolated from forest soil for bioremediation of mercury contamination in a substrate. Six fungal strains were selected based on their capability to grow in 25 mg/L Hg2+-contaminated potato dextrose agar plates. Fungal strain KRP1 showed the highest ratio of growth diameter, 0.831, thus was chosen for further observation.Identification based on colony and cell morphology carried out by 18S rRNA analysis gave a 98%match to Aspergillus flavus strain KRP1. The fungal characteristics in mercury(Ⅱ) contamination such as range of optimum pH, optimum temperature and tolerance level were 5.5–7 and 25–35℃ and 100 mg/L respectively. The concentration of mercury in the media affected fungal growth during lag phases. The capability of the fungal strain to remove the mercury(Ⅱ) contaminant was evaluated in 100 mL sterile 10 mg/L Hg2+-contaminated potato dextrose broth media in 250 mL Erlenmeyer flasks inoculated with 108spore/mL fungal spore suspension and incubation at 30℃ for 7 days. The mercury(Ⅱ) utilization was observed for flasks shaken in a 130 r/min orbital shaker(shaken) and nonshaken flasks(static) treatments. Flasks containing contaminated media with no fungal spores were also provided as control. All treatments were done in triplicate. The strain was able to remove 97.50%and 98.73% mercury from shaken and static systems respectively. A. flavus strain KRP1 seems to have potential use in bioremediation of aqueous substrates containing mercury(Ⅱ) through a biosorption mechanism. 关键词：土壤汞污染丝状真菌生物修复修复潜力森林土壤分离真菌菌株孢子悬浮液

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Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil

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