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Differential expression of microRNAs in dorsal root ganglia after sciatic nerve injury

查看全文 作　　者：Anjie [1]Lu;Zufa [1]Huang;Chaoyue [1]Zhang;Xianfang [2]Zhang;Jiuhong [2]Zhao;Haiying [2]Zhang;Quanpeng [2]Zhang;Song [1]Wu;Xinan [2]Yi 高影响力作者 机构地区：[1]Department of Orthopedics,the Third Xiangya Hospital,Central South University;[2]Neurosciences Research Department,Hainan Medical University高影响力机构 出　　处：《Neural Regeneration Research》索引2014年第9卷第10期,共10页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China,No.81160158 and 30860290 摘　　要：This study investigated the possible involvement of microRNAs in the regulation of genes that participate in peripheral neural regeneration.A microRNA microarray analysis was conducted and 23 microRNAs were identified whose expression was significantly changed in rat dorsal root ganglia after sciatic nerve transection.The expression of one of the downregulated microRNAs,microRNA-214,was validated using quantitative reverse transcriptase-PCR.MicroRNA-214 was predicted to target the 3′-untranslated region of Slit-Robo GTPase-activating protein 3.In situ hybridization verified that microRNA-214 was located in the cytoplasm of dorsal root ganglia primary neurons and was downregulated following sciatic nerve transection.Moreover,a combination of in situ hybridization and immunohistochemistry revealed that microRNA-214 and Slit-Robo GTPase-activating protein 3 were co-localized in dorsal root ganglion primary neurons.Western blot analysis suggested that Slit-Robo GTPase-activating protein 3 was upregulated in dorsal root ganglion neurons after sciatic nerve transection.These data demonstrate that microRNA-214 is located and differentially expressed in dorsal root ganglion primary neurons and may participate in regulating the gene expression of Slit-Robo GTPase-activating protein 3 after sciatic nerve transection. 关 键 词：背根神经节神经元 microRNA 差异表达 神经损伤 GTPASE RNA基因 微小RNA 坐骨神经
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