Effect of Honghua (Flos Carthami) on nitric oxide production in RAW 264.7 cells and α-glucosidase activity

查看全文 作　　者：Hui [1]Liao;Linda [2]Banbury;Hongping [3]Liang;Xiaomin [4]Wang;Xiaokai [1]Lü;Ling [5]Hu;Jin [6]Wu

机构地区：[1]Department of Pharmacy, Shanxi Provincial People's Hospital;[2]Southern Cross Plant Science, Southern Cross University;[3]Department of Clinical Laboratory, Shanxi Provincial People's Hospital;[4]Department of Pharmacy,Shanxi DaYi Hospital;[5]Department of Endocrinology, Shanxi Provincial People's Hospital;[6]Management of Hospital, Shanxi Medical University Second Hospital

出　　处：《Journal of Traditional Chinese Medicine》索引2014年第34卷第3期,共7页

基　　金：Supported by Shanxi Scholarship Council of China(No.2010-102) 摘　　要：OBJECTIVE: To study the effects of extracts from Honghua(Flos Carthami) on lipopolysaccharide induced nitric oxide(NO) production in RAW 264.7cells and the influence of the extracts on yeastα-glucosidase activity. The total flavonoid content of the extracts was also determined.METHODS: Cytotoxicity of the extracts to RAW264.7 cells was evaluated by the ATPlite? method.Inhibitory effects of the extracts on NO production were evaluated by Griess assay. Curcumin was used as a positive control. Screening of extracts for potential α-glucosidase inhibitors was done by a fluorometric assay.The assay was based on the hydrolysis of 4-methylumbelliferyl-a-D-glucopyranoside to form the fluorescent product, 4-methylumbelliferone. Acarbose was used as a positive control. The total flavonoid content was tested using kaempferol as the standard.RESULTS: There were significant inhibitory effects on NO production when the extracts were 25-100μg/mL(P<0.05) and curcumin was 2-4 μg/mL(P<0.001). The extracts showed an inhibitory effect onα-glucosidase activity at the concentrations of15.6-125 μg/mL with a half maximal(50%) inhibitory concentration(IC50) of(32.8±5.7) μg/mL, compared with the IC 50 of acarbose at(1.8±0.4) μg/mL.There was a significant difference between the two IC50values(P<0.001). The total content of flavonoids per gram of dried herb was1.14 mg.CONCLUSION:Honghua(Flos Carthami)showed in hibitory effects on NO production in activated RAW264.7 macrophage cells and an inhibitory effect on yeast α-glucosidase. There might be a relationship between these pharmacological effects and its flavonoid content. 关键词：细胞毒性一氧化氮红花 Α-葡萄糖苷酶抑制剂 NO生成抑制作用吡喃葡萄糖苷提取物

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Effect of Honghua (Flos Carthami) on nitric oxide production in RAW 264.7 cells and α-glucosidase activity

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