Immobilization of RGD Peptide onto the Surface of Apatite-Wollastonite Ceramic for Enhanced Osteoblast Adhesion and Bone Regeneration

查看全文 作　　者：[1]张翔;GU [1,2]Jianwen;ZHANG [3]Yue;TAN [1]Yanfei;ZHOU [1]Jiabei;[1]周大利

机构地区：[1]College of Materials Science and Engineering, Sichuan University;[2]The Military General Hospital of Chengdu PLA;[3]The First Affiliated Hospital of Gannan Medical University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》索引2014年第29卷第3期,共9页

基　　金：Supported in part by the Specialized Research Fund for theDoctoral Program of Higher Education of China(No.20060610024) 摘　　要：The arginine-glycine-aspartic(RGD) acid peptide was grafted to the surface of apatitewollastonite(AW) ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic cross linker AAPTS-E and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC). The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR) and X-ray photoelectron spectroscopy(XPS). The chemical analysis indicated that RGD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells(MG63) showed that modifying the AW surface with RGD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were signifi cantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit's femoral condyles. 关键词：成骨细胞样细胞陶瓷表面 RGD肽细胞黏附再生磷灰石傅里叶变换红外光谱 X射线光电子能谱

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Immobilization of RGD Peptide onto the Surface of Apatite-Wollastonite Ceramic for Enhanced Osteoblast Adhesion and Bone Regeneration

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