Improved dot blotting for small RNA detection

查看全文 作　　者：Li [1]Ren;Lianyong [2]Liu;Shuanggang [3]Hu;Zhaohui [1]Zhu;Hongling [1]Zhu;Junhua [1]Ma;Xuemei [1]Zhao;Xing [1]Wang;Chaobao [4]Zhang;Mingjun [1]Gu;Xiangqi [1]Li 机构地区：[1]Department of Endocrine,Shanghai Gongli Hospital,the Second Military Medical University,Shanghai 200135, China;[2]Department of Endocriology,Punan Hospital of Pudong New District,Shanghai 200125,China;[3]Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics,Center for Reproductive Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200135,China;[4]State Key Laboratory of Molecular Biology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,University of Chinese Academy of Sciences, Shanghai 200031,China 出　　处：《Acta Biochimica et Biophysica Sinica》索引2018年第50卷第12期,共3页 基　　金：the grants from the National Natural Science Foundation of China (No.81500655);the Pudong New Area Science and Technology Commission (No.PKJ2016-Y03);the Shanghai Health and Family Planning Commission (Nos. 201640177 and 201540393);the Discipline Construction Project of Health and Family Planning Commission of Pudong New Area (Nos.PWZxq2017-17,PWZxk2017-07,PWZzk2017-29,and PWZzb2017-22). 摘　　要：Non-coding small RNAs,such as small interfering RNA (siRNA), microRNA (miRNA),and piwi-interacting RNA (piRNA),have a common feature that their sizes always fall within a narrow,defined range of between 18 and 32nt [1,2].These tiny RNAs have revolutionized our comprehension of gene expression as key post-transcriptional regulators.Compared with long RNA,small RNA has a low abundance with very small size,making it difficult to be detected using traditional methods[3]. 关 键 词：RNA DETECTION