Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

查看全文 作　　者：Lingzi [1]Dai;Nian [1]Guo;Yaqin [1]Liu;Shanshan [1]Shen;Qiufu [2]Ge;Yuanjiang [1]Pan

机构地区：[1]Department of Chemistry, Zhejiang University;[2]Hangzhou Pharmaceutical Group Co., Ltd.

出　　处：《Chinese Chemical Letters》索引2019年第30卷第1期,共4页

基　　金：supported by the National Natural Science Foundation of China (Nos. 21327010, 21372199) 摘　　要：The binding between NL-101, a novel nitrogen mustard anti-cancer drug, with amino acids and peptides has been investigated by high performance liquid chromatography electrospray tandem mass spectrometry(HPLC/ESI-MS/MS). This study offers supporting data of the interaction among drug and amino acids and peptides, which could potentially explain the cytotoxic and mutagenic effects of the drug. Collision-induced dissociation(CID) experiment demonstrated that under the same collision energy, the amino group combined with NL-101 adducts are sensitive and often produce more fragment ions; the carboxyl group combined with NL-101 adducts are hard to break and display fewer fragment ions. In addition, when other group(like sulfhydryl group) of amino acids binds to NL-101, CID spectra show different fragmentation pattern. These differences could display structural information about the drug adducts and be utilized as location of the authentic binding sites. 关键词：High-performance liquid chromatography/ TANDEM mass SPECTROMETRY NL-101 AMINO ACIDS PEPTIDES Binding sites

相关文献

参考文献（21）
引证文献（4）
耦合文献（0）

参考文献(21)

引证文献(4)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

参考文献(21)

引证文献(4)

用户提示