以芳香胺玻璃为载体的固定化的胆固醇脂酶和胆固醇氧化酶

查看全文 作　　者：VoMalik S.Singh 等

机构地区：BiochemistryResearchLaboratory,DepartmentofBio-Sciences,M.D.University,Rohtak-24001(Haryana)India

出　　处：《生物工程学报》索引2002年第18卷第2期,共7页

摘　　要：Cholesterol esterase (Cease) from bovine pancreas and cholesterol oxidasc (COD) from Bravibacterium recombinant type have been innobilized individually and co-immobilized onto arylamine glass (pore diameter 55nm) through the process of diazotization. Cease and COD retained 92.65% and 85.54% of the initial activity with conjugation yields of 7.2 mg/g and 8.3mg/g support respectively when imnobilized individually on arylamine glass beads, but retained 89.58% of the initial activity with a conjugation yield of 2.9 mg/g support when co-immobilized on the same support. The effects of pH, temperature, time of incubation, substrate concentration, serum inorganic salts & metabolites, thermal stability, storage stability in cold and reusability on the immobilized enzymes were studied and compared with those of free enzymes. The analytic use of both individually immobilized and co-immobilized enzymes in discrete analysis of total and free cholesterol in serum is demonstrated. 关键词：胆固醇胆固醇脂酶胆固醇氧化酶固定化芳香胺玻璃血清胆固醇测定载体

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以芳香胺玻璃为载体的固定化的胆固醇脂酶和胆固醇氧化酶

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