Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach

查看全文 作　　者：YANG Dong [1]Hong;ZHANG Yuan [2,3]Yuan;DU Peng [2,3]Cheng;XU [1]Li;WANG Hai [2,3]Yin;HAN [2,3]Na;CHEN [2,3]Chen;GAO Zhan [1]Cheng

机构地区：[1]Department of Respiratory and Critical care medicine,Peking University People’s Hospital;[2]State Key Laboratory for Infectious Disease Prevention and Control,and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention;[3]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

出　　处：《Biomedical and Environmental Sciences》索引2014年第27卷第11期,共4页

基　　金：supported by grants from the Ministry of Science and Technology of China(National High-Tech R&D program:No.2006AA02Z4A9 and No.2012BAI05B02;12th Five-Year Plan Major Scientific and Technological Program:No.2012ZX10004-206);the Ministry of Health of China(Special Fund for Health-scientific Research in the Public Interest Program:No.201202011) 摘　　要：Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads(51.3%) were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis. 关键词：支气管肺泡灌洗液病原细菌基因组学方法快速鉴定焦磷酸测序物种宏 RDNA

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Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach

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