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Molecular characterization and developmental expression patterns of thyroid hormone receptors(TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

查看全文 作　　者：Qinqin [1,2]Lou;Yinfeng [1]Zhang;Dongkai [1]Ren;Haiming [1]Xu;Yaxian [1]Zhao;Zhanfen [1]Qin;Wuji [2]Wei 高影响力作者 机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences,Chinese Academy of Sciences;[2]College of Environment, Nanjing University of Technology高影响力机构 出　　处：《Journal of Environmental Sciences》索引2014年第26卷第10期,共11页高影响力期刊 基　　金：supported by the Public Welfare Research Project for Environmental Protection (No. 201109048);the National High Technology Research and Development Program (863) of China (No. 2012AA06A302);the National Natural Science Foundation of China (No. 21077125) 摘　　要：Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone(TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor(TR) agonist(T3) and antagonist(amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax.Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility. 关 键 词：甲状腺激素受体 表达模式 黑斑蛙 拮抗剂 激动剂 TRS 特征和 物种特异性
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