The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

查看全文 作　　者：BAI Li-[1,2]jing;JU Hui-[1,3]ming;MU Yu-[1]lian;YANG Shu-[1]lin;REN Hong-[1]yan;AO [1]Hong;WANG Chu-[2]duan;LI [1]Kui

机构地区：[1]State Key Laboratory for Animal Nutrition, Ministry of Agriculture/Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences;[2]College of Animal Science, China Agricultural University;[3]College of Veterinary Medicine, Yangzhou University

出　　处：《Journal of Integrative Agriculture》索引2014年第13卷第12期,共9页

基　　金：supported by the National Transgenic Breeding Project of China (2011ZX08010-003);the National Natural Science Foundation of China (31272405, 31101683) 摘　　要：Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coliappA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pc DNA-app A-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressedappA and MxA showed a gene integration rate of 8.89%(16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by theappA gene(P<0.05); these animals also exhibited a strong anti-viral phenotype. 关键词：转基因小鼠 MXA蛋白 APPA 精原细胞 A型体内介导合作

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The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

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