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In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration

查看全文 作　　者：LIN Yong [1]Xin;DING Zhi [2]Yong;ZHOU Xiao [1]Bin;LI Si [3]Tao;XIE De [4]Ming;LI Zhi [1]Zhong;SUN Guo [1]Dong 高影响力作者 机构地区：[1]Department of Orthopedics, the First Affiliated Hospital of Jinan University;[2]Department of Traumatology, Hui Zhou Municipal Central Hospital;[3]Department of Pediatrics, the Sixth Affiliated Hospital of Sun Yat-sen University;[4]Department of Biomedical Engineering, College of Life Science and Technology, Jinan University高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2015年第28卷第1期,共12页高影响力期刊 基　　金：financially supported by the National Natural Science Foundation of China,No.31070862;Science and Technology Plan of Guangzhou,No.12C32071662;Research Foundation of Guangdong Provincial Bureau of Traditional Chinese Medicine,No.2013113;scientific research and cultivating Foundation of the First Clinical Medical College of Jinan University,No.2012103 and No.2013208 摘　　要：Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects. Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope(SEM), transmission electron microscope(TEM), and fourier transform infrared spectroscopy(FTIR), human umbilical cord mesenchymal stem cells(h UC-MSCs) culture and animal experiments. Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. h UC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds(P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds. Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction. 关 键 词：玉米醇溶蛋白 羟基磷灰石 支架材料 PLGA 软骨形成 体内 傅里叶变换红外光谱 扫描电子显微镜
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