Gas chromatography-mass spectrometry analysis of essential oils from five parts of Chaihu(Radix Bupleuri Chinensis)

查看全文 作　　者：Jie [1,2]Meng;Xingfu [1,2]Chen;Wenyu [1,2]Yang;Jiuhua [1,2]Song;Yu [1,2]Zhang;Zhifei [1,2]Li;Xingwang [3]Yang;Zhanguo [3]Yang

机构地区：[1]Department of Pharmaceutical Botany,College of Agronomy, Sichuan Agricultural University;[2]Key Laboratory of Crop Physiology, Ecology and Cultivation inSouthwest, Ministry of Agriculture;[3]Production Division, Sichuan Depeiyuan Traditional Chinese Medicine Technology Development Co., Ltd.

出　　处：《Journal of Traditional Chinese Medicine》索引2014年第34卷第6期,共8页

基　　金：Breeding Research Project of Chinese Medical Herbs in Sichuan Province:Study on the breeding of new Chaihu variety and cultivation and the related techniques(No.2011NZ0098-12-11);China Spark Program in 2010(No.2010GA810056) 摘　　要：OBJECTIVE: To analyze the essential oils from flowers, leaves, stems, roots, and fruits of Chaihu(Radix Bupleuri Chinensis).METHODS: We extracted essential oils from different parts of Chaihu(Radix Bupleuri Chinensis) using a steam distillation method. The essential oils were analyzed by gas chromatography-mass spectrometry(GC-MS). Data were collected in full scan mode(m/z 60-600). Volatile components were identified based on their retention indices and by comparing their mass spectra with those in the National Institute of Standards and Technology 2005 database assisted by tandem mass spectrometry information. The relative content of each constituent wasdetermined by area normalization.RESULTS: We identified 111 components, of which12 were common to all 5 parts, 30 were found only in roots, 14 were found only in flowers, 6 were found only in leaves, 4 were found only in stems,and 17 were found only in fruits.CONCLUSION: Our results show that the stems,flowers, leaves, and fruits of Chaihu(Radix Bupleuri Chinensis)contain a high concentration of essential oils, and that the exact composition of the essential oils differs among the plant parts. To develop new medicines and make full use of the Chaihu(Radix Bupleuri Chinensis)resource, it is important to characterize the essential oils from different parts of the plant. In future research, it will be important to determine the pharmacological effects of the various components and the essential oil mixtures. 关键词：气相色谱质谱图柴胡精油羊草光谱分析水蒸气蒸馏法美国国家标准

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Gas chromatography-mass spectrometry analysis of essential oils from five parts of Chaihu(Radix Bupleuri Chinensis)

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