Comparative proteomic and metabolomic analyses reveal mechanisms of improved cold stress tolerance in bermudagrass (Cynodon dactylon(L.) Pers.) by exogenous calcium

查看全文 作　　者：Haitao [1]Shi;Tiantian [1,2]Ye;Bao [1,2]Zhong;Xun [1,2]Liu;Zhulong [1]Chan

机构地区：[1]Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,the Chinese Academy of Sciences;[2]University of Chinese Academy of Sciences

出　　处：《Journal of Integrative Plant Biology》索引2014年第56卷第11期,共16页

基　　金：supported by 'the Hundred Talents Program,' the Knowledge Innovative Key Program of the Chinese Academy of Sciences (54Y154761O01076 and Y329631O0263) to Z.C.;the National Natural Science Foundation of China (31200194);the outstanding young talent program of Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture (Y352811O03);Youth Innovation Promotion Association of the Chinese Academy of Sciences to H.S. 摘　　要：As an important second messenger, calcium is involved in plant cold stress response, including chilling(<20 °C) and freezing(<0 °C). In this study, exogenous application of calcium chloride(CaCl2) improved both chilling and freezing stress tolerances, while ethylene glycol‐bis‐(b‐aminoethyl) ether‐N,N,N,N‐tetraacetic acid(EGTA) reversed CaCl2 effects in bermudagrass(Cynodon dactylon(L.) Pers.).Pysiological analyses showed that CaCl2 treatment alleviated the reactive oxygen species(ROS) burst and cell damage triggered by chilling stress, via activating antioxidant enzymes,non‐enzymatic glutathione antioxidant pool, while EGTA treatment had the opposite effects. Additionally, comparative proteomic analysis identified 51 differentially expressed proteins that were enriched in redox, tricarboxylicacid cycle,glycolysis, photosynthesis, oxidative pentose phosphate pathway, and amino acid metabolisms. Consistently, 42 metabolites including amino acids, organic acids, sugars, and sugar alcohols were regulated by CaCl2 treatment under control and cold stress conditions, further confirming the Researchcommon modulation of CaCl2 treatment in carbon metabolites and amino acid metabolism. Taken together, this study reported first evidence of the essential and protective roles of endogenous and exogenous calcium in bermudagrass response to cold stress, partially via activation of the antioxidants and modulation of several differentially expressed proteins and metabolic homeostasis in the process of cold acclimation. 关键词：比较蛋白质组学狗牙根外源钙冷应激耐受机制学分代谢组氨基酸代谢

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Comparative proteomic and metabolomic analyses reveal mechanisms of improved cold stress tolerance in bermudagrass (Cynodon dactylon(L.) Pers.) by exogenous calcium

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