Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

查看全文 作　　者：Yuuki [1]Sakai;Shin-Ichiro [2]Inoue;Akiko [3]Harada;Ken-Ichiro [4]Shimazaki;Shingo [1]Takagi

机构地区：[1]Department of Biological Sciences, Graduate School of Science, Osaka University;[2]Division of Biological Science, Graduate School of Science, Nagoya University;[3]Department of Biology, Osaka Medical College;[4]Department of Biology, Faculty of Science, Kyushu University

出　　处：《Journal of Integrative Plant Biology》索引2015年第57卷第1期,共13页

基　　金：supported by the Japanese Ministry of Education, Culture, Sports, Science and Technology (grant Nos. 19039020 and 20570037 to S.T.) 摘　　要：In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces'chloroplast de-anchoring', a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de-anchoring is known induced within 1 min of irradiation with high-fluence-rate blue light specifically, preceding the commencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response. In search of the responsible photoreceptors,immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-k Da exist in the plasma-membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120-k Da polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner. The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologs(Vallisneria PHOTOTROPIN1 and PHOTOTROPIN2)expressed in leaves were isolated. We propose that calcium-regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisneria. 关键词：叶表皮细胞叶绿体光诱导锚固蓝色水生被子植物细胞内钙库磷酸化

相关文献

参考文献（71）
引证文献（1）
耦合文献（3）

参考文献(71)

引证文献(1)

耦合文献(3)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

参考文献(71)

引证文献(1)

耦合文献(3)

用户提示