Polysaccharides Purified from Wild Cordyceps Activate FGF2/FGFR1c Signaling

查看全文 作　　者：ZENG [1]Yangyang;HAN [1]Zhangrun;YU [1]Guangli;HAO [1]Jiejie;ZHANG [1]Lijuan

机构地区：[1]Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience &Glycotechnology, School of Medicine and Pharmacy, Ocean University of China

出　　处：《Journal of Ocean University of China》索引2015年第14卷第1期,共7页

基　　金：supported by the National Natural Science Foundation of China (Grant No. 91129706) 摘　　要：Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes in their living environment may be a sharp decrease in salt concentration. It is established that sulfated polysaccharides interact with hundreds of signaling molecules and facilitate many signaling transduction pathways, including fibroblast growth factor(FGF) and FGF receptor signaling pathway. The disappearance of sulfated polysaccharides in fungi and plants on land might indicate that polysaccharides without sulfation might be sufficient in facilitating protein ligand/receptor interactions in low salinity land. Recently, it was reported that plants on land start to synthesize sulfated polysaccharides in high salt environment, suggesting that fungi might be able to do the same when exposed in such environment. Interestingly, Cordyceps, a fungus habituating inside caterpillar body, is the most valued traditional Chinese Medicine. One of the important pharmaceutical active ingredients in Cordyceps is polysaccharides. Therefore, we hypothesize that the salty environment inside caterpillar body might allow the fungi to synthesize sulfated polysaccharides. To test the hypothesis, we isolated polysaccharides from both lava and sporophore of wild Cordyceps and also from Cordyceps militaris cultured without or with added salts. We then measured the polysaccharide activity using a FGF2/FGFR1 c signaling-dependent Ba F3 cell proliferation assay and found that polysaccharides isolated from wild Cordyceps activated FGF2/FGFR signaling, indicating that the polysaccharides synthesized by wild Cordyceps are indeed different from those by the cultured mycelium. 关键词：信号转导通路硫酸多糖冬虫夏草野生成纤维细胞生长因子激活生物合成纯化

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Polysaccharides Purified from Wild Cordyceps Activate FGF2/FGFR1c Signaling

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