Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties

查看全文 作　　者：Palaniselvam [1]Kuppusamy;Solachuddin [2]J.A.Ichwan;Narasimha Reddy [3]Parine;Mashitah [1]M.Yusoff;Gaanty Pragas [1]Maniam;Natanamurugaraj [1]Govindan

机构地区：[1]Biomaterial and Biosensor Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang;[2]Kulliyyah of Dentistry, International Islamic University Malaysia;[3]Genome Research Chair, Department of Biochemistry, College of Science, King Saud University

出　　处：《Journal of Environmental Sciences》索引2015年第27卷第3期,共7页

基　　金：supported by Universiti Malaysia Pahang through the internal research grant nos. GRS 130336 and RDU 120302 摘　　要：In this present study, we reported broccoli(Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles(NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24–38 nm for gold and 30–45 nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally,the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes(sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/m L respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens. 关键词：金纳米颗粒乙醇提取物生物合成生物学特性场发射扫描电子显微镜紫外可见分光光度计傅里叶变换红外光谱甘蓝

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Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties

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