Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs

查看全文 作　　者：Qiumei [1,2]Feng;Hongyuan [1,2]Chen;Jingjuan [1,2]Xu

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science;[2]School of Chemistry and Chemical Engineering, Nanjing University

出　　处：《Science China Chemistry》索引2015年第58卷第5期,共9页

基　　金：supported by the National Basic Research Program of China(2012CB932600);the National Natural Science Foundation of China(21327902,21475058,21121091) 摘　　要：A novel disposable paper-based bipolar electrode(BPE) array is fabricated for multiplexed electrochemiluminescence(ECL) detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each consisting of six sensing cells and two reporting cells patterned using hydrophobic wax. A hairpin structure DNA assembled on the cathodes of BPEs hybridizes with Pt nanoparticles(NPs) labeled probe DNA in the presence of complementary target DNA. The introduction of Pt NPs catalyzes the reduction of dissolved O2 at cathodes and induces an enhanced ECL signal from Ru(bpy)32+/tripropylamine(TPr A) at the anodes of BPEs. The dissolved O2 lost in reduction reaction could be promptly replenished due to the relatively large contact area of the paper-based cells with air, which ensures the stability of ECL signal. This obtained paper-based BPE array sensor showed excellent performances for the multiplexed analysis of the syphilis(Treponema pallidum) gene, the immunodeficiency virus gene(HIV) and hepatitis B virus gene(HBV). 关键词：化学发光检测电极阵列多路复用 DNA 一次性病原双极纸质

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Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs

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