Identification and characterization of a novel isoform of hepatopoietin

查看全文 作　　者：Jun Lu Wei-Min Cai,Institute of Infectious Disease,First Affiliated Hospital,Medical SchooI,Zhejiang University,Hangzhou 310003 China Wang-Xiang Xu Yi-Qun Zhan Xiao-Lin Cui Fu-Chu He Xiao-Ming Yang,Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China

出　　处：《World Journal of Gastroenterology》索引2002年第8卷第2期,共4页

基　　金：the National Natural Science Foundation of China,No.39830440 摘　　要：AIM: To isolate a novel isoform of human HPO (HPO-205)human fetal liver Marathon-reedy cDNA andcharacterize its primary biological function.METHODS: 5'-RACE (rapid amplification of cDNA 5' ends)was used to isolate a novel isoform of hHPO in this paperThe constructed pcDNAHPO-205, pcDNAHPO and pcDNA eukaryotic expression vectors were respectively transfectedby lipofectamine method and the stimulation of DNAsynthesis was observed by 3H-TdR incorporation assay.Proteins extracted from different cells were analyzed byWestern blot.RESULTS: A novel isoform of hHPO (HPO-205) encoding a205 amino acid ORF corresponding to a translatedproduction of 23 kDa was isolated and distinguished fromthe previous HPO that lacked the N-terminal 80 amino acids.The dnse-dspendent stimulation of DNA synthesis of HepG2hepatoma cells by HPO-205 demonstrated its similarbiological activity with HPO in vitro. The level of MAPK(Mitogen-activated protein kinase) phnsphorylarion byWestern blot analysis revealed that HPO-205 might have thestronger activity of stimulating hepatic cell proliferation thanthat of HPO.CONCLUSION: A novel isoform of hHPO (HPO-205) wasisolated from hepatic-derived cells. The comparison of HPO-205 and HPO will lead to a new insight into the structure andfunction of hHPO, and provide the new way of thinking todeeply elucidate the biological roles of HPO/ALR. 关键词：肝生成素A 对碘氧基本甲醚识别特性

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Identification and characterization of a novel isoform of hepatopoietin

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