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Development and Application of an RT-PCR Method for Differentiation of PEDV Variant Strains and Classical Strains

查看全文 作　　者：Qin [1]Yibin;Lu [1]Bingxia;Zhao [1]Wu;He [1]Ying;Li [1,2]Yingying;Li [1]Bin;Liang [1]Jiaxing;Liang [1]Baozhong;Su [1]Qianlian;Jiang [1]Dongfu;Zhou [1]Yingning 高影响力作者 机构地区：[1]Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Animal Vaccines and New Technology;[2]College of Animal Science and Technology,Guangxi University高影响力机构 出　　处：《Animal Husbandry and Feed Science》索引2015年第7卷第1期,共5页高影响力期刊 基　　金：Supported by the Scientific Research Project of Guangxi Bureau of Livestock,Fisheries and Veterinary Services(12049031);the Systemic Research Project of Guangxi Key Laboratory of Animal Vaccines and New Technology(12-071-28-A-5);the Basal Research Fund of Guangxi(13-1) 摘　　要：In order to establish a rapid RT-PCR assay for detection of porcine epidemic diarrhea virus(PEDV),a pair of special primers was designed based on S gene sequence of PEDV published in Gen Bank.After optimization of the reaction system,a rapid RT-PCR method was established.Results showed that a fragment of 826 bp was successfully amplified only from the variant PEDV by RT-PCR,while the expected target fragment could not be amplified from TGEV,porcine rotavirus,porcine kobuvirus,PRRSV,PRV,CSFV,PPV.Sensitivity test of RT-PCR indicated that only 11.3 pg nuclear acids could be detected accurately and rapidly.A total of 123 samples collected from different farms in Guangxi Province were detected by the established RT-PCR,the positive rate of PEDV was 67.5%,and the positive rate of new PEDV was 86.7%(72/83).Therefore,the RT-PCR could be used as an effective tool for differentiating diagnosis of the highly pathogenic PEDV in epidemiological investigations. 关 键 词：猪流行性腹泻病毒 PCR检测方法 RT-PCR检测 变异株系 RT-PCR法 猪传染性胃肠炎 PEDV 应用
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