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Electroacupuncture pretreatment exhibits antidepressive effects by regulating hippocampal proteomics in rats with chronic restraint stress

查看全文 作　　者：Zhuo [1]Guo;Ya [1]Tu;Tian-wei [1]Guo;Yun-chu [2]Wu;Xue-qin [1]Yang;Lan [1]Sun;Xin-jing [3]Yang;Wen-yue [1]Zhang;Yu [1]Wang;Xu-hui [1]Zhang 高影响力作者 机构地区：[1]Beijing University of Chinese Medicine;[2]Shijiazhuang Hospital of Traditional Chinese Medicine;[3]Beijing Rehabilitation Hospital of Capital Medical University高影响力机构 出　　处：《Neural Regeneration Research》索引2015年第10卷第8期,共7页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China,No.81373729 摘　　要：The clinical effect of electroacupuncture on depression is widely recognized. However, the signal transduction pathways and target proteins involved remain unclear. In the present study, rat models of chronic restraint stress were used to explore the mechanism by which electroacupuncture alleviates depression. Rats were randomly divided into control, model, and electroacupuncture groups. Chronic restraint stress was induced in the model and electroacupuncture groups by restraining rats for 28 days. In the electroacupuncture group, electroacupuncture pretreatment at Baihui(GV20) and Yintang(GV29) acupoints was performed daily(1 m A, 2 Hz, discontinuous wave, 20 minutes) prior to restraint for 28 days. Open field tests and body weight measurements were carried out to evaluate the depressive symptoms at specific time points. On day 28, the crossing number, rearing number, and body weights of the model group were significantly lower than those in the control group. Behavior test results indicated that rat models of depressive-like symptoms were successfully established by chronic restraint stress combined with solitary raising. On day 28, an isobaric tag for a relative and absolute quantitation-based quantitative proteomic approach was performed to identify differentially expressed proteins in hippocampal samples obtained from the model and electroacupuncture groups. The potential function of these differential proteins was predicted through the use of the Cluster of Orthologous Groups of proteins(COG) database. Twenty-seven differential proteins(uncharacteristic proteins expected) were selected from the model and electroacupuncture groups. In addition to unknown protein functions, COG are mainly concentrated in general prediction function, mechanism of signal transduction, amino acid transport and metabolism groups. This suggests that electroacupuncture improved depressive-like symptoms by regulating differential proteins, and most of these related proteins exist in nerve cells. 关 键 词：定量蛋白质组学 电针治疗 大鼠模型 束缚应激 抑郁症 预处理 慢性 海马
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