Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole(Cynoglossus semilaevis)

查看全文 作　　者：Tian-Zi [1]WANG;Ai [1,2]SUN;Na [1,2]WANG;Zhong-Kai [1]CUI;Song-Lin [1]CHEN;Zhen-Xia [1]SHA

机构地区：[1]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;[2]Function Laboratory for Marine Fisheries Science and Food Production Processes,National Lab for Ocean Science and Technology

出　　处：《Zoological Research》索引2015年第36卷第5期,共5页

基　　金：supported by grants from the National Natural Science Foundation of China(31130057);the State863 High-Technology R&D Project of China(2012AA10A408);the Taishan Scholar Project Fund of Shandong Province of China;the Special Scientific Research Funds for the Central Non-profit Institutes,Chinese Academy of Fishery Sciences(2013A0402) 摘　　要：An astroglial cell line was established from the brain of half smooth tongue sole(Cynoglossus semilaevis)and was designated as CSAC.CSAC shows the morphological homogeneity of epithelial cells.The cell identity was tested by the presence of glial fibrillary acidic protein(GFAP),which was revealed by RT-PCR and immunofluorescence.The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES,antibiotics,20%fetal bovine serum,2-Mercaptoethanol(2-Me) and basic fibroblast growth factor.Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number(2n=42).The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein(GFP) reporter plasmids.The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells. 关键词：星形胶质细胞半滑舌鳎细胞系鉴定机构碱性成纤维细胞生长因子大脑胶质纤维酸性蛋白荧光显示

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Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole(Cynoglossus semilaevis)

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