Cloning and Sequence Analysis of IGFBP-7 Gene in Sheep

查看全文 作　　者：Mingliang [1]ZHOU;Pinggui [1]YANG;Dengjun [2]WU;Xiangyu [3]ZHANG 机构地区：[1]Sichuan Academy of Grassland Science;[2]College of Animal Sci-Technology,Sichuan Agricultural University;[3]Sichuan Academy of Animal Science 出　　处：《Agricultural Biotechnology》索引2015年第4卷第6期,共5页 基　　金：Supported by Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture(201003061);Key Project of Livestock and Poultry Breeding of Sichuan Province(01NG029-18) 摘　　要：In this study,full-length CDS sequence of IGFBP-7 gene was cloned from Liangshan semi-fine wool sheep with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-7 gene in Liangshan semi-fine wool sheep was 846 bp in length,encoding282 amino acids. The CDS sequence shared 99%,95% and 90% homology with bovine,human and rat,respectively; the amino acid sequence shared 98%,93%and 89%,respectively. The GenB ank accession number was FJ589640. 1. The amino acid molecular weight of IGFBP-7 was 29. 0 kD,and the theoretical isoelectric point( pI) was 8. 25. The result of phylogenetic analysis showed that IGFBP-7 gene in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine,goat and other mammals,and distant phylogenetic relationships with Danio rerio and Haliotis diversicolor. IGFBP-7 gene had uniformly distributed hydrophobic and hydrophilic regions,harboring one signal peptide,two transmembrane regions,16 phosphorylation sites,four N-glycosylation sites and one O-glycosylation site. The result of secondary structure analysis showed that the random coil,α-helix and β-sheet regions accounted for 64. 89%,19. 86% and 15. 25%,respectively. The result of tertiary structure analysis showed that IGFBP-7 harbors an IGFBP_N domain and an Ig-like domain. This study provided scientific basis for further investigating the function of IGFBP-7 gene in sheep. 关 键 词：克隆分析 基因 绵羊 RT-PCR方法 GENBANK 半细毛羊 系统发育分析 二级结构分析